Anti-RNF25 antibody (ab51475)
Key features and details
- Rabbit polyclonal to RNF25
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF25 antibody
See all RNF25 primary antibodies -
Description
Rabbit polyclonal to RNF25 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
A synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab51475 was affinity purified from monospecific antiserum by immunoaffinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNF25 antibody (ab51475)All lanes : Anti-RNF25 antibody (ab51475) at 1/1000 dilution
Lane 1 : HEK293 lysate from
cells transfected with human
RNF25
Lane 2 : HEK293 lysate from
cells transfected with mouse RNF25
Lane 3 : HEK293 lysate from cells transfected with empty vector
Predicted band size: 51 kDa
Observed band size: 54 kDa why is the actual band size different from the predicted?
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Immunoprecipitation - Anti-RNF25 antibody (ab51475)1ug ab51475 was used in immunoprecipitation of 20 µg HEK293 cell lysate over-expressing HA-tagged human RNF25 (lane 1) or vector only control (lane 2). The precipitated complex was loaded onto a gel, followed by electrophoresis and transfer to nitrocellulose. The membrane was probed with anti-HA tag antibody.
