Anti-RNF25 antibody (ab89281)
Key features and details
- Mouse polyclonal to RNF25
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF25 antibody
See all RNF25 primary antibodies -
Description
Mouse polyclonal to RNF25 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length Human RNF25 protein (NP_071898.2).
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Positive control
- HeLa cell lysate. HeLa nuclear extract. RNF25 transfected 293T cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNF25 antibody (ab89281)Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Western blot - Anti-RNF25 antibody (ab89281)Anti-RNF25 antibody (ab89281) at 1 µg/ml + HeLa Nuclear extract at 50 µg
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Western blot - Anti-RNF25 antibody (ab89281)All lanes : Anti-RNF25 antibody (ab89281) at 1 µg/ml
Lane 1 : RNF25 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
