Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11305(2)] to RNF21/IFP1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free
    See all RNF21/IFP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11305(2)] to RNF21/IFP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab250142 is the carrier-free version of ab180130 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab250142 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. This product was previously labelled as RNF21

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)
    Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)
    Anti-RNF21/IFP1 antibody [EPR11305(2)] (ab180130) at 1/2000 dilution + Jurkat cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa



    This data was developed using ab180130, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)
    Flow Cytometry - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)
    This data was developed using ab180130, the same antibody clone in a different buffer formulation.Flow cytometry analysis of K562 cells labeling RNF21/IFP1 using ab180130 at 1/60 dilution. A Goat anti rabbit IgG (FITC) at 1/75 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Isotype control: Rabbit IgG.
  • Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)
    Western blot - Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)
    Anti-RNF21/IFP1 antibody [EPR11305(2)] (ab180130) at 1/10000 dilution + K562 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 57 kDa



    This data was developed using ab180130, the same antibody clone in a different buffer formulation.

     

  • Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)
    Anti-RNF21/IFP1 antibody [EPR11305(2)] - BSA and Azide free (ab250142)

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