Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab226469)
Key features and details
- Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Coxsackie Adenovirus Receptor/hCAR antibody
See all Coxsackie Adenovirus Receptor/hCAR primary antibodies -
Description
Rabbit polyclonal to Coxsackie Adenovirus Receptor/hCAR -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human Coxsackie Adenovirus Receptor/hCAR aa 300-335. The exact sequence is proprietary. (NP_001329.1).
Database link: P78310 -
Positive control
- WB: HeLa whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma tissue.
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General notes
This product was previously labelled as Coxsackie Adenovirus Receptor
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226469 was affinity purified using an epitope specific to Coxsackie Adenovirus Receptor/hCAR immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab226469)All lanes : Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab226469) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 40 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab226469)Coxsackie Adenovirus Receptor/hCAR was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226469 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226469 at 0.4 µg/ml.
Lane 1: ab226469 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coxsackie Adenovirus Receptor/hCAR antibody (ab226469)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for Coxsackie Adenovirus Receptor/hCAR using ab226469 at 1/200 dilution in immunohistochemical analysis.
Detection: Peroxidase Substrate.
