Antibodies, Proteins, Kits and Reagents for Life Science

Product name

Anti-LMX1b antibody [EPR24053-161] - BSA and Azide free
See all LMX1b primary antibodies
Description

Rabbit monoclonal [EPR24053-161] to LMX1b - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: WB, Dot blot, IP, IHC-Pmore details
Unsuitable for: Flow Cyt (Intra),ICC or IHC-Fr
Species reactivity

Reacts with: Mouse, Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Mouse hypothalamus, Rat E17 embryo, HeLa transfected with mouse LMX1b full length expression vector containing a myc-His-tag®, E.coli extracts containing his-tagged mouse LMX1a full length recombinant protein. IHC-P: Mouse hypothalamus, Mouse kidney, Rat hypothalamus, Rat kidney and Rat E14.5 embryo tissues. IP: HEK-293T transfected with mouse LMX1b expression vector containing a myc-His-tag®.
General notes
ab281937 is the carrier-free version of ab259926. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab281937 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C.
Storage buffer

Constituent: 100% PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR24053-161
Isotype

IgG

Western blot - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

All lanes : Anti-LMX1b antibody [EPR24053-161] (ab259926) at 1/1000 dilution

Lane 1 : Mouse hypothalamus tissue lysate
Lane 2 : Mouse stomach tissue lysate
Lane 3 : Mouse ovary tissue lysate
Lane 4 : Rat hypothalamus tissue lysate
Lane 5 : Rat E17 embryo tissue lysate
Lane 6 : Rat stomach tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution (VeriBlot for IP secondary antibody(HRP))

Observed band size: 45 kDa why is the actual band size different from the predicted?

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: stomach and ovary (PMID: 24056967).

This blot was developed using a higher sensitivity ECL substrate.

Exposure time: 3 minutes
Western blot - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

All lanes : Anti-LMX1b antibody [EPR24053-161] (ab259926) at 1/1000 dilution

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) transfected with mouse LMX1b full length expression vector containing a myc-His-tag®, whole cell lysate at 10 µg
Lane 2 : E.coli extracts containing his-tagged mouse LMX1a full length recombinant protein at 20 µl

Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Lane 2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration: 5% NFDM/TBST

This antibody has a weak cross-reactivity with LMX1a.

Exposure time: 5.5 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse hypothalamus tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on mouse hypothalamus (PMID: 27407143). The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the glomerulus of mouse kidney. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat hypothalamus tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on rat hypothalamus. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the glomerulus of rat kidney (PMID: 24785190). The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat E14.5 embryo tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Nuclear staining on the spinal cord of rat E14.5 embryo. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labelling LMX1b with ab259926 at 1/2000 (0.278 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: almost no staining on mouse stomach. The section was incubated with ab259926 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
Immunoprecipitation - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.

LMX1b was immunoprecipitated from 0.35 mg HEK-293T (human embryonic kidney) transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate 10 ug with ab259926 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259926 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: HEK-293T (human embryonic kidney) transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate 10 ug

Lane 2: ab259926 IP in HEK-293T transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab259926 in HEK-293T transfected with mouse LMX1b expression vector containing a myc-His-tag®, whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 32 seconds
Dot Blot - Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)

This data was developed using ab259926, the same antibody clone in a different buffer formulation.
Dot blot analysis of LMX1b using ab259926 at 1:1000 (0.556 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:100,000 dilution.

Exposure time: 3 minutes
Blocking and diluting buffer and concentration: 5% NFDM/TBST
Anti-LMX1A + LMX1b antibody [EPR24053-161] - BSA and Azide free (ab281937)