Anti-RNF20 antibody (ab32629)
Key features and details
- Rabbit polyclonal to RNF20
- Suitable for: WB, ICC/IF
- Reacts with: Chicken, Human
- Isotype: IgG
Overview
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Product name
Anti-RNF20 antibody
See all RNF20 primary antibodies -
Description
Rabbit polyclonal to RNF20 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Chicken, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Human RNF20 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab32628) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB (8) Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 114 kDa).ICC/IF (6) Use a concentration of 1 µg/ml.Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 120 kDa (predicted molecular weight: 114 kDa).ICC/IF
Use a concentration of 1 µg/ml.Target
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Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes. Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer cells leading to its proteasome-mediated degradation. -
Tissue specificity
Expressed in the normal brain and also in malignant gliomas (at protein level). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the BRE1 family.
Contains 1 RING-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 427310 Chicken
- Entrez Gene: 56254 Human
- Entrez Gene: 109331 Mouse
- Omim: 607699 Human
- SwissProt: Q5ZLS3 Chicken
- SwissProt: Q5VTR2 Human
- SwissProt: Q5DTM8 Mouse
- Unigene: 729085 Human
see all -
Alternative names
- BRE1 A antibody
- BRE1 antibody
- BRE1 E3 ubiquitin ligase homolog antibody
see all
Images
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Western blot - Anti-RNF20 antibody (ab32629)All lanes : Anti-RNF20 antibody (ab32629) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Lanes 2-6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody (ab32629)This image is courtesy of an Abreview submitted by Dr Kirk McManusab32629 (1/500) staining RNF20 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI to highlight the nucleus (red). Please refer to abreview for further experimental details. -
Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody (ab32629)ICC/IF image of ab32629 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32629, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml. -
Western blot - Anti-RNF20 antibody (ab32629)Image courtesy of an anonymous Abreview.All lanes : Anti-RNF20 antibody (ab32629) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from Ntera2 cells
Lane 2 : Whole cell lysate prepared from Ntera2 cells + siRNA against RNF20 for 48 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
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Western blot - Anti-RNF20 antibody (ab32629)Anti-RNF20 antibody (ab32629) at 1 µg/ml + Liver (Chicken) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab32629 has been referenced in 17 publications.
- Suraweera A et al. COMMD4 functions with the histone H2A-H2B dimer for the timely repair of DNA double-strand breaks. Commun Biol 4:484 (2021). PubMed: 33875784
- Mani C et al. The multifunctional protein PACS-1 is required for HDAC2- and HDAC3-dependent chromatin maturation and genomic stability. Oncogene 39:2583-2596 (2020). PubMed: 31988453
- Fiore D et al. N6-Isopentenyladenosine Inhibits Colorectal Cancer and Improves Sensitivity to 5-Fluorouracil-Targeting FBXW7 Tumor Suppressor. Cancers (Basel) 11:N/A (2019). PubMed: 31569395
- Wang JH et al. Activation of the mammalian target of rapamycin signaling pathway underlies a novel inhibitory role of ring finger protein 182 in ventricular remodeling after myocardial ischemia-reperfusion injury. J Cell Biochem N/A:N/A (2018). PubMed: 30450663
- Shah VJ & Maddika S CRL7SMU1 E3 ligase complex-driven H2B ubiquitylation functions in sister chromatid cohesion by regulating SMC1 expression. J Cell Sci 131:N/A (2018). PubMed: 29507117
Images
-
Western blot - Anti-RNF20 antibody (ab32629)All lanes : Anti-RNF20 antibody (ab32629) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 :Jurkat whole cell lysate (ab7899)
Lane 3 :A-431 whole cell lysate (ab7909)
Lane 4 :HEK-293 whole cell lysate (ab7902)
Lane 5 : MCF-7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Lanes 2-6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
-
Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody (ab32629) This image is courtesy of an Abreview submitted by Dr Kirk McManusab32629 (1/500) staining RNF20 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI to highlight the nucleus (red). Please refer to abreview for further experimental details.
-
Immunocytochemistry/ Immunofluorescence - Anti-RNF20 antibody (ab32629)ICC/IF image of ab32629 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab32629, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 1µg/ml.
-
Western blot - Anti-RNF20 antibody (ab32629) Image courtesy of an anonymous Abreview.All lanes : Anti-RNF20 antibody (ab32629) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from Ntera2 cells
Lane 2 : Whole cell lysate prepared from Ntera2 cells + siRNA against RNF20 for 48 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated goat polyclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 120 kDa why is the actual band size different from the predicted?
Exposure time: 20 seconds
-
Western blot - Anti-RNF20 antibody (ab32629)Anti-RNF20 antibody (ab32629) at 1 µg/ml + Liver (Chicken) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 114 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Exposure time: 20 minutes
