Anti-RNF20 antibody (ab70495)
Key features and details
- Rabbit polyclonal to RNF20
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RNF20 antibody
See all RNF20 primary antibodies -
Description
Rabbit polyclonal to RNF20 -
Host species
Rabbit -
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human RNF20
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Positive control
- Whole cell lysate from HeLa and 293T cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RNF20 antibody (ab70495)All lanes : Anti-RNF20 antibody (ab70495) at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 114 kDa
Observed band size: 140 kDa why is the actual band size different from the predicted?
Additional bands at: 171 kDa, 280 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-RNF20 antibody (ab70495)Detection of Human RNF20 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70495 at 3 µg/mg lysate. Subsequent RNF20 WB detection was performed using ab70495 at 1 µg/ml.
