Griess reagent (ab234044)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 1 nmol/well
Overview
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Product name
Griess reagent -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
1 nmol/well -
Assay time
1h 30m -
Product overview
Griess Reagent Kit ab234044 uses a classic protocol for the estimation of nitrite in biological samples.
Nitrite is reduced to nitrogen oxide using Griess Reagent I. Nitrogen oxide then reacts with Griess Reagent II forming a stable product that can be detected by its absorbance at 540 nm.
The two-step assay is simple, fast and can detect nitrite levels as low as 1 nmol/well.
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Platform
Microplate reader
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 200 tests 500 tests 1000 tests Griess Reagent I 1 x 10ml 1 x 25ml 1 x 50ml Griess Reagent II 1 x 10ml 1 x 25ml 1 x 50ml Nitrite Assay Buffer 1 x 30ml 1 x 75ml 1 x 150ml Nitrite Standard 1 vial 1 vial 1 vial
Images
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Nitrite standard curve
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Nitrite concentration in pooled human serum and HeLa nuclear extractsHuman serum was deproteinized using 10 kDa columns. Filtrate (20 µl, undiluted) was collected and assayed according to kit protocols. HeLa nuclear cells were homogenized using Nitrite Assay Buffer (100 µg protein).