Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10020] to SF3A3 - BSA and Azide free
  • Suitable for: ICC, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-SF3A3 antibody [EPR10020] - BSA and Azide free
    See all SF3A3 primary antibodies

  • Description

    Rabbit monoclonal [EPR10020] to SF3A3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab249286 is the carrier-free version of ab156873. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249286 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR10020

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)

    This data was developed using ab156873, the same antibody clone in a different buffer formulation.

    ab156873 showing +ve staining in Human normal brain. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)

    This data was developed using ab156873, the same antibody clone in a different buffer formulation.

    ab156873 showing +ve staining in Human normal kidney. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)

    This data was developed using ab156873, the same antibody clone in a different buffer formulation.

    ab156873 showing +ve staining in Human normal colon. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    This data was developed using ab156873, the same antibody clone in a different buffer formulation.ab156873 showing +ve staining in Human cervical carcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    This data was developed using ab156873, the same antibody clone in a different buffer formulation.ab156873 showing +ve staining in Human breast carcinoma. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Western blot - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Western blot - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    All lanes : Anti-SF3A3 antibody [EPR10020] (ab156873) at 1/1000 dilution

    Lane 1 : Human fetal liver tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 59 kDa



    This data was developed using ab156873, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Immunocytochemistry - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    This data was developed using ab156873, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Hela cells labeling SF3A3 with ab156873 at 1/100 dilution.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    This data was developed using ab156873, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin-fixed, paraffin-embedded lung carcinoma tissue labelling SF3A3 with ab156873 at 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)
    Anti-SF3A3 antibody [EPR10020] - BSA and Azide free (ab249286)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.