Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)
![Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)](https://www.abcam.com/ps/products/250/ab250475/Images/ab250475-407868-anti-dus2l-antibody-epr14042-bsa-and-azide-free-western-blot.jpg "Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14042] to DUS2L - BSA and Azide free
- Suitable for: ICC, IP, Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-DUS2L antibody [EPR14042] - BSA and Azide free
See all DUS2L primary antibodies -
Description
Rabbit monoclonal [EPR14042] to DUS2L - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IP, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250475 is the carrier-free version of ab181262. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250475 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR14042 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)All lanes : Anti-DUS2L antibody [EPR14042] (ab181262) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 55 kDaThis data was developed using ab181262, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)](https://www.abcam.com/ps/products/250/ab250475/Images/ab250475-3-anti-dus2l-antibody-epr14042-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)This data was developed using ab181262, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling DUS2L with ab181262 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue). -
![Flow Cytometry - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)](https://www.abcam.com/ps/products/250/ab250475/Images/ab250475-2-anti-dus2l-antibody-epr14042-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)This data was developed using ab181262, the same antibody clone in a different buffer formulation.Flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling DUS2L with ab181262 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution. -
![Immunoprecipitation - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)](https://www.abcam.com/ps/products/250/ab250475/Images/ab250475-1-anti-dus2l-antibody-epr14042-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)This data was developed using ab181262, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from A549 cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181262 at 1/50 dilution.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution. -
![Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)](https://www.abcam.com/ps/products/250/ab250475/Images/ab250475-5-benefits-of-recombinant-antibodies.png)
Anti-DUS2L antibody [EPR14042] - BSA and Azide free (ab250475)