Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)
![Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)](https://www.abcam.com/ps/products/223/ab223148/Images/ab223148-3-ab207429274885207429IHC1.jpg "Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20357] to Ribonuclease 3/ECP - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
-
Product name
Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free
See all Ribonuclease 3/ECP primary antibodies -
Description
Rabbit monoclonal [EPR20357] to Ribonuclease 3/ECP - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
-
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: GST-His-tagged human Ribonuclease 3/ECP recombinant protein (aa28-160); Human fetal lung and fetal spleen lysates. IHC-P: Human Hodgkin’s lymphoma, spleen and colon tissues.
-
General notes
ab223148 is the carrier-free version of ab207429 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab223148 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Ribonuclease 3
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20357 -
Isotype
IgG
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)
Immunohistochemical analysis of paraffin-embedded human Hodgkin’s lymphoma tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human Hodgkin’s lymphoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207429).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)](https://www.abcam.com/ps/products/223/ab223148/Images/ab223148-2-ab207429274884207429IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human spleen [PMID: 11034418].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207429).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)](https://www.abcam.com/ps/products/223/ab223148/Images/ab223148-1-ab207429274883207429IHC3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Ribonuclease 3/ECP with ab207429 at 1/8000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on eosinophils and neutrophils of human colon [PMID: 11034418].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207429).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)](https://www.abcam.com/ps/products/223/ab223148/Images/ab223148-4-benefits-of-recombinant-antibodies.png)
Anti-Ribonuclease 3/ECP antibody [EPR20357] - BSA and Azide free (ab223148)
