Anti-REST / NRSF antibody (ab21635)
Key features and details
- Rabbit polyclonal to REST / NRSF
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-REST / NRSF antibody
See all REST / NRSF primary antibodies -
Description
Rabbit polyclonal to REST / NRSF -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Mouse REST/ NRSF aa 1050 to the C-terminus (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17042)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
-
Compatible Secondaries
-
Immunizing Peptide (Blocking)
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB (2) Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 121 kDa (predicted molecular weight: 121 kDa).Application notesIs unsuitable for IHC-P.Target
-
Function
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. -
Tissue specificity
Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. -
Sequence similarities
Contains 9 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 5978 Human
- Entrez Gene: 19712 Mouse
- Entrez Gene: 83618 Rat
- Omim: 600571 Human
- SwissProt: Q13127 Human
- SwissProt: Q155C6 Mouse
- SwissProt: Q8VIG1 Mouse
- SwissProt: O54963 Rat
see all -
Alternative names
- Neural Restrictive Silencer Factor antibody
- Neural-restrictive silencer factor antibody
- Neuron Restrictive Silencer Factor antibody
see all
Images
-
Western blot - Anti-REST / NRSF antibody (ab21635)All lanes : Anti-REST / NRSF antibody (ab21635) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate withMouse REST / NRSF peptide (ab17042) at 1 µg/ml
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate withMouse REST / NRSF peptide (ab17042) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 121 kDa
Additional bands at: 53 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Protocols
References (20)
ab21635 has been referenced in 20 publications.
- Kawamura M et al. Loss of nuclear REST/NRSF in aged-dopaminergic neurons in Parkinson's disease patients. Neurosci Lett 699:59-63 (2019). PubMed: 30684677
- Liu Q et al. Decreased hyperpolarization-activated cyclic nucleotide-gated channels are involved in bladder dysfunction associated with spinal cord injury. Int J Mol Med 41:2609-2618 (2018). PubMed: 29436607
- Yu Y et al. NRSF/REST levels are decreased in cholangiocellular carcinoma but not hepatocellular carcinoma compared with normal liver tissues: A tissue microarray study. Oncol Lett 15:6592-6598 (2018). PubMed: 29725406
- Wang R et al. The differentiation of human MSCs derived from adipose and amniotic tissues into insulin-producing cells, induced by PEI@Fe3O4 nanoparticles-mediated NRSF and SHH silencing. Int J Mol Med 42:2831-2838 (2018). PubMed: 30132574
- Wang J et al. Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos. Cell 175:1887-1901.e18 (2018). PubMed: 30550787
Images
-
Western blot - Anti-REST / NRSF antibody (ab21635)All lanes : Anti-REST / NRSF antibody (ab21635) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate withMouse REST / NRSF peptide (ab17042) at 1 µg/ml
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate withMouse REST / NRSF peptide (ab17042) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 121 kDa
Observed band size: 121 kDa
Additional bands at: 53 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
