Anti-REST / NRSF antibody - C-terminal (ab227885)
Key features and details
- Rabbit polyclonal to REST / NRSF - C-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-REST / NRSF antibody - C-terminal
See all REST / NRSF primary antibodies -
Description
Rabbit polyclonal to REST / NRSF - C-terminal -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Recombinant fragment within Human REST/ NRSF (C terminal). The exact sequence is proprietary.
Database link: Q13127 -
Positive control
- WB: U-87 MG whole cell and nuclear extracts; HeLa whole cell and nuclear lysates; HeLa cell extract. IHC-P: Human lung tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-REST / NRSF antibody - C-terminal (ab227885)All lanes : Anti-REST / NRSF antibody - C-terminal (ab227885) at 1/500 dilution
Lane 1 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa5% SDS-PAGE gel.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-REST / NRSF antibody - C-terminal (ab227885)Paraffin-embedded human lung tissue stained for REST / NRSF using ab227885 at 1/400 dilution in immunohistochemical analysis.
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Western blot - Anti-REST / NRSF antibody - C-terminal (ab227885)All lanes : Anti-REST / NRSF antibody - C-terminal (ab227885) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 122 kDa5 % SDS-PAGE gel.
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Western blot - Anti-REST / NRSF antibody - C-terminal (ab227885)All lanes : Anti-REST / NRSF antibody - C-terminal (ab227885) at 1/500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : REST / NSRF knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 122 kDa5% SDS-PAGE gel.
