Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] (ab94805)
![Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] (ab94805)](https://www.abcam.com/ps/products/94/ab94805/Images/ab94805-337142-anti-respiratory-syncytial-virus-m2-1-protein-antibody-rsv5h5-western-blot.jpg "Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] (ab94805)")
Key features and details
- Mouse monoclonal [RSV5H5] to Respiratory Syncytial Virus M2-1 Protein
- Suitable for: ELISA, WB, IP, ICC/IF
- Reacts with: Respiratory syncytial virus
- Isotype: IgG1
Overview
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Product name
Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] -
Description
Mouse monoclonal [RSV5H5] to Respiratory Syncytial Virus M2-1 Protein -
Host species
Mouse -
Specificity
This antibody has broad specificity for Respiratory Syncytial Virus of both sub-groups A and B -
Tested applications
Suitable for: ELISA, WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Respiratory syncytial virus -
Immunogen
Tissue, cells or virus corresponding to Respiratory Syncytial Virus M2-1 Protein. Respiratory Syncytial Virus strain A2 infected HeLa cells.
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General notes
Fusion partner: PS-NS/1-Ag4 Recommended growth conditions: Growth medium RPMI with 25mM Hepes, 2mM additional glutamine and 10% heat inactivated foetal calf serumReproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
RSV5H5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Respiratory Syncytial Virus M2-1 Protein antibody [RSV5H5] (ab94805) Jeong et al PLoS One. 2015 Jun 24;10(6):e0130517. doi: 10.1371/journal.pone.0130517. eCollection 2015. S1 File. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
RSV mutants show expected patterns of G, F, and M2-1 expression.
Western blotting of Vero cell lysates infected with RSV ΔG, ΔG C-term, and wild type (MSA1).
(Figure C shown) Detection of M2-1 using anti-M2-1 antibody ab94805.
Cell lysates from virus cultures grown for 6 days on Vero cells were subjected to SDS-PAGE, transferred to membranes and probed with anti-M2-1 antibody (Abcam, ab94805), and incubated with a secondary goat anti-mouse alkaline phosphatase conjugate. Anti-G western blot was done with rabbit anti-RSV-G polyclonal and incubated with goat anti-rabbit secondary antibody (Abcam, ab97048). Bands were visualized using BCIP/NBT. The molecular weight marker used was Precision Plus Protein Dual Color Standards.