Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RANKL antibody [101] - BSA and Azide free (ab276057)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [101] to RANKL - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-RANKL antibody [101] - BSA and Azide free
    See all RANKL primary antibodies

  • Description

    Rabbit monoclonal [101] to RANKL - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment corresponding to Human RANKL aa 63-244.
    Database link: O14788-2

  • Positive control

    • WB: HEK-293T membrane lysate. IP: Raji whole cell lysate.

Properties

Images

  • Immunoprecipitation - Anti-RANKL antibody [101] (ab276057)
    Immunoprecipitation - Anti-RANKL antibody [101] (ab276057)

    RANKL was immunoprecipitated from 0.5 mg of Raji (human Burkitt's lymphoma cell line) whole cell lysate with 0.5 µL of ab276057 and 15 µl of 50 % Protein G agarose. Western blot was performed from the immunoprecipitate using ab276057 at 1/500 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibody at 1/5000 dilution.

    Developed using the odssey technique.
    Performed under reducing conditions.

  • Western blot - Anti-RANKL antibody [101] (ab276057)
    Western blot - Anti-RANKL antibody [101] (ab276057)
    Anti-RANKL antibody [101] - BSA and Azide free (ab276057) at 1/500 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) membrane lysate at 30 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

    Predicted band size: 35 kDa



    Developed using the Odyssey technique.
    Performed under reducing conditions.

  • Anti-RANKL antibody [101] - BSA and Azide free (ab276057)
    Anti-RANKL antibody [101] - BSA and Azide free (ab276057)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.