Anti-FANCM antibody (ab95014)
Key features and details
- Rabbit polyclonal to FANCM
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-FANCM antibody
See all FANCM primary antibodies -
Description
Rabbit polyclonal to FANCM -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
A region within N terminal amino acids 1-50 (MSGRQRTLFQ TWGSSISRSS GTPGCSSGTE RPQSPGSSKA PLPAAAEAQL) of Human FANCM (NP_065988.1).
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Positive control
- HeLa whole cell lysate (ab150035)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab95014 was affinity purified using an epitope specific to FANCM immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-FANCM antibody (ab95014)All lanes : Anti-FANCM antibody (ab95014) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 227 kDa
Exposure time: 10 seconds
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Immunoprecipitation - Anti-FANCM antibody (ab95014)ab95014, at 0.4 µg/ml, detecting FANCM in HeLa whole cell lysate by WB following IP.
Lane 1: ab95014 at 10 µg/mg of lysate
Lane 2: IP with an antibody which recognizes a downstream epitope of FANCM
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 10 seconds
