Anti-RANK antibody [64C1385] (ab13918)
![Anti-RANK antibody [64C1385] (ab13918)](https://www.abcam.com/ps/products/13/ab13918/Images/ab13918-283167-anti-rank-antibody-64c1385-immunohistochemistry.png "Anti-RANK antibody [64C1385] (ab13918)"){kind=icon}
Key features and details
- Mouse monoclonal [64C1385] to RANK
- Suitable for: IHC-P, IP
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-RANK antibody [64C1385]
See all RANK primary antibodies -
Description
Mouse monoclonal [64C1385] to RANK -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanIP Mouse
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Immunogen
Recombinant fragment within Human RANK. The exact sequence is proprietary. Recombinant fragment containing the extracellular domain (amino acids 33-208) of human RANK.
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Positive control
- WB: RAW (a mouse macrophage monocyte). IHC-P: Human T cell lymphoma and skin tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
64C1385 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)
Formaldehyde-fixed, paraffin-embedded human skin tissue stained for RANK using ab13918 at 1/50 dilution in immunohistochemical analysis.
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![Immunoprecipitation - Anti-RANK antibody [64C1385] (ab13918)](https://www.abcam.com/ps/products/13/ab13918/Images/ab13918-21413-anti-rank-antibody-64c1385-immunoprecipitation.gif)
Immunoprecipitation - Anti-RANK antibody [64C1385] (ab13918)Detection of RANK in RAW cells. Lane 1: mouse cell line. Lane 2 & 3: IP/Western blot analysis of RANK. RANK protein from RAW cell lysate was immunoprecipitated either with control antibody (lane 2) or ab13918 (lane 3), and detected with ab13918.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)](https://www.abcam.com/ps/products/13/ab13918/Images/ab13918-21412-anti-rank-antibody-64c1385-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RANK antibody [64C1385] (ab13918)IHC image of ab13918 staining in human t cell lymphoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13918, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
