Anti-RANK antibody [8H8B2] (ab233817)
Key features and details
- Mouse monoclonal [8H8B2] to RANK
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
-
Product name
Anti-RANK antibody [8H8B2]
See all RANK primary antibodies -
Description
Mouse monoclonal [8H8B2] to RANK -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment corresponding to Human RANK aa 30-212. Extracellular domain. Expressed in E.coli.
Database link: Q9Y6Q6 -
Positive control
- WB: Recombinant human RANK (aa 30-212) protein; RANK (aa 30-212)-hIgG-Fc-transfected HEK-293 cell lysate. Flow cyt: HeLa and HL-60 cells.
-
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8H8B2 -
Isotype
IgG1 -
Research areas
Images
-
Anti-RANK antibody [8H8B2] (ab233817) at 1/500 dilution + Recombinant human RANK (aa 30-212) protein
Predicted band size: 66 kDaExpected recombinant MW is 46 kDa.
-
All lanes : Anti-RANK antibody [8H8B2] (ab233817) at 1/500 dilution
Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : RANK (aa 30-212)-hIgG-Fc-transfected HEK-293 cell lysate
Predicted band size: 66 kDa
-
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RANK with ab233817 at 1/200 dilution (green) compared with a negative control (red).
-
Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling RANK with ab233817 at 1/200 dilution (green) compared with a negative control (red).