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Anti-RANK antibody [8H8B2] (ab233817)

Anti-RANK antibody [8H8B2] (ab233817)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [8H8B2] to RANK
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG1

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Overview

  • Product name

    Anti-RANK antibody [8H8B2]
    See all RANK primary antibodies
  • Description

    Mouse monoclonal [8H8B2] to RANK
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human, Recombinant fragment
  • Immunogen

    Recombinant fragment corresponding to Human RANK aa 30-212. Extracellular domain. Expressed in E.coli.
    Database link: Q9Y6Q6

  • Positive control

    • WB: Recombinant human RANK (aa 30-212) protein; RANK (aa 30-212)-hIgG-Fc-transfected HEK-293 cell lysate. Flow cyt: HeLa and HL-60 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    8H8B2
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Innate Immunity
    • Cytokines
    • TNF Superfamily
    • Signal Transduction
    • Adapters
    • Transmembrane
    • Signal Transduction
    • Growth Factors/Hormones
    • TNF
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • Structures
    • Bone
    • Cardiovascular
    • Atherosclerosis
    • Vascular Inflammation
    • Inflammatory mediators

Images

  • Western blot - Anti-RANK antibody [8H8B2] (ab233817)
    Western blot - Anti-RANK antibody [8H8B2] (ab233817)
    Anti-RANK antibody [8H8B2] (ab233817) at 1/500 dilution + Recombinant human RANK (aa 30-212) protein

    Predicted band size: 66 kDa



    Expected recombinant MW is 46 kDa.

  • Western blot - Anti-RANK antibody [8H8B2] (ab233817)
    Western blot - Anti-RANK antibody [8H8B2] (ab233817)
    All lanes : Anti-RANK antibody [8H8B2] (ab233817) at 1/500 dilution

    Lane 1 : Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
    Lane 2 : RANK (aa 30-212)-hIgG-Fc-transfected HEK-293 cell lysate

    Predicted band size: 66 kDa

  • Flow Cytometry - Anti-RANK antibody [8H8B2] (ab233817)
    Flow Cytometry - Anti-RANK antibody [8H8B2] (ab233817)

    Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling RANK with ab233817 at 1/200 dilution (green) compared with a negative control (red).

  • Flow Cytometry - Anti-RANK antibody [8H8B2] (ab233817)
    Flow Cytometry - Anti-RANK antibody [8H8B2] (ab233817)

    Flow cytometric analysis of HL-60 (human promyelocytic leukemia cell line) cells labeling RANK with ab233817 at 1/200 dilution (green) compared with a negative control (red).

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-RANK antibody [8H8B2] (ab233817)

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    Anti-RANK antibody (ab222215)

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    Anti-RANK antibody (ab200369)

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    Anti-RANK antibody [EPR4740(N)] (ab182158)

    Applications: WB

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    Anti-RANK antibody [64C1385] (ab13918)

    Applications: IHC-P, IP

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