Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)
Key features and details
- Rabbit polyclonal to Rpa2 (phospho S4 + S8)
- Suitable for: IP, IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Rpa2 (phospho S4 + S8) antibody -
Description
Rabbit polyclonal to Rpa2 (phospho S4 + S8) -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Orangutan
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Immunogen
Synthetic peptide within Human Rpa2 (phospho S4 + S8). The exact sequence is proprietary. NP_002937.1
Database link: P15927 -
Positive control
- WB: HeLa cell lysate treated with 100 µM etoposide. IHC-P: Human lung cancer and mouse colon carcinoma tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)
Formaldehyde-fixed asynchronous HeLa cells. Untreated cells (left) and etoposide-treated cells (right) labeling Rpa2 using ab264116 at 1/1000 dilution (Red). A Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1/100. Counterstained with an Alex Fluor® phalloidin (Green).
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Western blot - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)All lanes : Anti-Rpa2 (phospho S4 + S8) antibody (ab264116) at 0.1 µg/ml
Lane 1 : HeLa cells treated with 100 µM etoposide (+)
Lane 2 : HeLa cells mock treated (-)
Lysates/proteins at 50 µg per lane.Detection: Chemiluminescence with an exposure time of 75 seconds.
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Immunoprecipitation - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with etoposide and/or Lambda PP or mock treated. RPA32 (S4/S8) was immunoprecipitated using a different rabbit anti-RPA32 antibody. For blotting immunoprecipitated Phospho RPA32 (S4/S8), ab264116 was used at 0.1 μg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Lane 1: ab264116 treated with etoposide and Lambda PP.
Lane 2: ab264116 treated with etopside.
Lane 3: Control IgG treated with etopside. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)Formalin-fixed, paraffin-embedded mouse colon carcinoma tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rpa2 (phospho S4 + S8) antibody (ab264116)Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Rpa2 using ab264116 at 1/1000 dilution in immunohistochemical analysis. Detection: DAB