Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3043] to Rab4 - Early Endosome Marker
  • Suitable for: WB, IP, Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Rab4 antibody [EPR3043] - Early Endosome Marker
    See all Rab4 primary antibodies

  • Description

    Rabbit monoclonal [EPR3043] to Rab4 - Early Endosome Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7, PC12, Neuro 2a, 293T, SH SY5Y and Human fetal brain lysates; ICC/IF: HeLa cells. Flow Cyt: HeLa cells. IP: MCF7 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified ab109009 at 1:170 dilution (10 µg/ml). Cells were fixed in 100% Methanol. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Rab4 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Human fetal brain lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab109009 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab109009 was shown to specifically react with Rab4 when Rab4 knockout samples were used. Wild-type and Rab4 knockout samples were subjected to SDS-PAGE. ab109009 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Purified)

    Lane 1 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
    Lane 2 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
    Lane 3 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates

    Lysates/proteins at 1/15 dilution per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 24 kDa

  • Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Immunoprecipitation - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    ab109009 (purified) at 1:80 dilution (2µg) immunoprecipitating Rab4 in MCF7 whole cell lysate.
    Lane 1 (input): MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab109009 & MCF7 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109009 in MCF7 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Flow Cytometry - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Rab4 with Purified ab109009 at 1:200 dilution (1 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) labelling Rab4 with purified ab109009 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

  • Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Western blot - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    All lanes : Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009) at 1/1000 dilution

    Lane 1 : MCF7 cell lysate
    Lane 2 : PC12 cell lysate
    Lane 3 : Neuro 2a cell lysate
    Lane 4 : 293T cell lysate
    Lane 5 : SH SY5Y cell lysate
    Lane 6 : Human fetal brain lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Immunocytochemistry/ Immunofluorescence - Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    ab109009 at 1/500 dilution staining Rab4 in HeLa by Immunofluorescence.
  • Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)
    Anti-Rab4 antibody [EPR3043] - Early Endosome Marker (ab109009)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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