Anti-DEK antibody [EPR11034] - BSA and Azide free (ab249362)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11034] to DEK - BSA and Azide free
- Suitable for: ICC, IHC-P, Flow Cyt, WB, IP
- Reacts with: Human
Overview
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Product name
Anti-DEK antibody [EPR11034] - BSA and Azide free
See all DEK primary antibodies -
Description
Rabbit monoclonal [EPR11034] to DEK - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, IHC-P, Flow Cyt, WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab249362 is the carrier-free version of ab166624 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab249362 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11034 -
Isotype
IgG -
Research areas
Images
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This data was developed using ab166624, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling DEK with ab166624 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
All lanes : Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDaThis data was developed using ab166624, the same antibody clone in a different buffer formulation.
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This data was developed using ab166624, the same antibody clone in a different buffer formulation.Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling DEK (red) with ab166624 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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This data was developed using ab166624, the same antibody clone in a different buffer formulation.
Immunoprecipitation of DEK in pellet from HeLa cell lysate using ab166624 at 1/10 dilution, followed by western blot detection of DEK using as secondary an HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.
Anti-DEK antibody [EPR11034] (ab166624) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate at 10 µg
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This data was developed using ab166624, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling DEK with ab166624 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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