Anti-PTIP antibody (ab70434)
Key features and details
- Rabbit polyclonal to PTIP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PTIP antibody
See all PTIP primary antibodies -
Description
Rabbit polyclonal to PTIP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide corresponding to Human PTIP (N terminal). Synthetic peptide mapping to a region between residues 1 and 50 of Human PTIP, using the numbering given in Jowsey, Doherty and Rouse, 2004, J. Biol. Chem. 279(53):55562-55569
Database link: Q6ZW49-6 -
Positive control
- Whole cell lysate from 293T cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Lanes 1 & 4 & 7 : Anti-PTIP antibody (ab70434) at 0.25 µg/ml
Lanes 2 & 5 & 8 : Anti-PTIP antibody (ab70434) at 0.1 µg/ml
Lanes 3 & 6 & 9 : Anti-PTIP antibody (ab70434) at 0.025 µg/ml
Lanes 1 & 4 & 7 : Whole cell lysate from 293T cells at 5 µg
Lanes 2 & 5 & 8 : Whole cell lysate from 293T cells at 15 µg
Lanes 3 & 6 & 9 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 118 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
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30ug of whole cell lysate from 293T cells were immunoprecipitated using ab70434 at 10, 5 and 1ug/mg of lysate respectively in lanes 1, 2 and 3. For the subsequent blot ab70434 was used at 0.25ug/ml.