Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)
![Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)](https://www.abcam.com/ps/products/248/ab248537/Images/ab248537-449209-recombinant-anti-pten-antibody-epr4408-76-western-blot-hela-lysates.jpg "Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4408-76] to PTEN - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PTEN antibody [EPR4408-76] - BSA and Azide free
See all PTEN primary antibodies -
Description
Rabbit monoclonal [EPR4408-76] to PTEN - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Human PTEN.
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Positive control
- WB: HAP1 and HeLa cell lysates.
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General notes
ab248537 is the carrier-free version of ab133532. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248537 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR4408-76 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)All lanes : Anti-PTEN antibody [EPR4408-76] (ab133532) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PTEN knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab133532).
Lanes 1- 2: Merged signal (red and green). Green - ab133532 observed at 47 kDa. Red - Anti-Vinculin antibody [VIN-54] observed at 124 kDa.
ab133532 was shown to react with PTEN in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255419 (knockout cell lysate ab263829) was used. Wild-type HeLa and PTEN knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab133532 and Anti-Vinculin antibody [VIN-54] overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)](https://www.abcam.com/ps/products/248/ab248537/Images/ab248537-454493-anti-pten-antibody-epr4408-76-bsa-and-azide-free-western-blot-hela-wt-hela-pten-knockout-hap1.jpg)
Western blot - Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)All lanes : Anti-PTEN antibody [EPR4408-76] (ab133532) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : PTEN knockout HeLa cell lysate
Lane 3 : HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab133532).
Lanes 1 - 3: Merged signal (red and green). Green - ab133532 observed at 47 kDa. Red - loading control, ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.
ab133532 was shown to react with PTEN in wild-type HeLa cells in western blot. The bands observed in PTEN knockout cell line ab255419 (PTEN knockout cell lysate ab263829) below 47 kDa may represent truncated forms and cleaved fragments. This has not been investigated further. HeLa wild-type and PTEN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab133532 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
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![Western blot - Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)](https://www.abcam.com/ps/products/248/ab248537/Images/ab248537-449541-anti-pten-antibody-epr4408-76-western-blot-hap1-human.jpg)
Western blot - Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)Lanes 1-2 : Anti-PTEN antibody [EPR4408-76] (ab133532) at 1/10000 dilution
Lanes 3-4 : Anti-GAPDH antibody [6C5] - Loading Control (ab8245) at 1/2000 dilution
Lanes 1 & 3 & 5 : PTEN knockout HAP1 cell lysate
Lanes 2 & 4 & 6 : Wild-type HAP1 cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 47 kDaThis data was developed using the same antibody clone in a different buffer formulation (ab133532).
Lanes 1 and 2: Green signal from target - ab133532 observed at 47 kDa
Lanes 3 and 4: Red signal from loading control - ab8245 observed at 37 kDa
Lanes 5 and 6: Merged (red and green) signal
ab133532 was shown to specifically react with PTEN in wild-type HAP1 cells. No band was observed when PTEN knockout samples were used. Wild-type and PTEN knockout samples were subjected to SDS-PAGE, ab133532 and ab8245 (loading control to GAPDH) were diluted to 1/10,000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging. -
![Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)](https://www.abcam.com/ps/products/248/ab248537/Images/ab248537-2-benefits-of-recombinant-antibodies.png)
Anti-PTEN antibody [EPR4408-76] - BSA and Azide free (ab248537)