Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-p60 katanin antibody [EPR5071] - BSA and Azide free (ab247943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5071] to p60 katanin - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-p60 katanin antibody [EPR5071] - BSA and Azide free
    See all p60 katanin primary antibodies

  • Description

    Rabbit monoclonal [EPR5071] to p60 katanin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247943 is the carrier-free version of ab111881 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247943 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-p60 katanin antibody [EPR5071] - BSA and Azide free (ab247943)
    Western blot - Anti-p60 katanin antibody [EPR5071] - BSA and Azide free (ab247943)
    All lanes : Anti-p60 katanin antibody [EPR5071] (ab111881) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 56 kDa



    This data was developed using ab111881, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-p60 katanin antibody [EPR5071] - BSA and Azide free (ab247943)
    Immunocytochemistry - Anti-p60 katanin antibody [EPR5071] - BSA and Azide free (ab247943)
    This data was developed using ab111881, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling p60 katanin with ab111881 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing staining on the centrosome of Hela cells. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red). The negative controls are as follows:- -ve control 1: ab111881 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution. -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Anti-p60 katanin antibody [EPR5071] - BSA and Azide free (ab247943)
    Anti-p60 katanin antibody [EPR5071] - BSA and Azide free (ab247943)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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