Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free (ab250367)
![Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free (ab250367)](https://www.abcam.com/ps/products/250/ab250367/Images/ab250367-2-anti-proteasome-activator-subunit-4-psme4-antibody-epr13576-b-bsa-and-azide-free-flow-cytometry.jpg "Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free (ab250367)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13576(B)] to Proteasome Activator Subunit 4/PSME4 - BSA and Azide free
- Suitable for: Flow Cyt, WB
- Reacts with: Human
Overview
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Product name
Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free
See all Proteasome Activator Subunit 4/PSME4 primary antibodies -
Description
Rabbit monoclonal [EPR13576(B)] to Proteasome Activator Subunit 4/PSME4 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250367 is the carrier-free version of ab181128 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab250367 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as Proteasome Activator Subunit 4
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13576(B) -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free (ab250367)This data was developed using ab181128, the same antibody clone in a different buffer formulation.Flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling Proteasome Activator Subunit 4/PSME4 with ab181128 at 1/210 dilution (red) or a Rabbit monoclonal IgG (negative) (green) followed by Goat anti rabbit IgG (FITC) secondary used at 1/150 dilution.
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![Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free (ab250367)](https://www.abcam.com/ps/products/250/ab250367/Images/ab250367-399350-anti-proteasome-activator-subunit-4-psme4-antibody-epr13576-b-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free (ab250367)All lanes : Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - N-terminal (ab181128) at 1/10000 dilution
Lane 1 : HepG2 lysate
Lane 2 : 293T lysate
Lane 3 : Jurkat lysate
Lane 4 : HeLa lysate
Lane 5 : K562 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 211 kDaThis data was developed using ab181128, the same antibody clone in a different buffer formulation.
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![Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free (ab250367)](https://www.abcam.com/ps/products/250/ab250367/Images/ab250367-3-benefits-of-recombinant-antibodies.png)
Anti-Proteasome Activator Subunit 4/PSME4 antibody [EPR13576(B)] - BSA and Azide free (ab250367)