Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19839] to Prosurfactant Protein C - Low endotoxin, Azide free
  • Suitable for: IP, IHC-Fr, WB, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free
    See all Prosurfactant Protein C primary antibodies

  • Description

    Rabbit monoclonal [EPR19839] to Prosurfactant Protein C - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-Fr, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.

  • General notes

    ab222929 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 15186480].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse spleen. [PMID: 15186480]. Counter stained with Hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)
    Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Prosurfactant Protein C with ab211326  at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 23967208].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

  • Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)
    Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)

    SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse lung lysate, 10ug (Input).

    Lane 2: ab211326 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    The banding pattern represents pro- and mature forms of SP-C.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab211326).

  • Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)
    Anti-Prosurfactant Protein C antibody [EPR19839] - Low endotoxin, Azide free (ab222929)

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