Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19839] to Prosurfactant Protein C
  • Suitable for: WB, IHC-Fr, IP, IHC-P
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Prosurfactant Protein C antibody [EPR19839]
    See all Prosurfactant Protein C primary antibodies

  • Description

    Rabbit monoclonal [EPR19839] to Prosurfactant Protein C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-Fr
    Mouse
    IHC-P
    Mouse
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
    Immunohistochemistry (Frozen sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Prosurfactant Protein C with ab211326  at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 23967208].

    The nuclear counterstain is DAPI (blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 15186480].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
    Western blot - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
    All lanes : Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Mouse spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The banding pattern in Lane 1 represents pro- and mature forms of SP-C.

    Negative control: mouse spleen. [PMID: 15186480].

  • Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
    Immunoprecipitation - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)

    SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

    Lane 1: Mouse lung lysate, 10ug (Input).

    Lane 2: ab211326 IP in mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 5 seconds.

    The banding pattern represents pro- and mature forms of SFTPC.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Negative control: no staining on mouse spleen. [PMID: 15186480]. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
    Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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