CPM, Thiol-reactive fluorescent probe (ab145275)
Key features and details
- Thiol-reactive fluorescent probe.
- CAS Number: 76877-33-3
- Purity: > 95%
- Soluble in DMSO
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
CPM, Thiol-reactive fluorescent probe -
Description
Thiol-reactive fluorescent probe. -
Alternative names
- 7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin
- N-(4-(7-diethylamino-4-methylcoumarin-3-yl) phenyl) maleimide
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Biological description
Widely used blue fluorescent thiol-reactive dye. Essentially non-fluorescent until it reacts with thiols, making it possible to quantify thiols without a separation step. Good energy acceptor from tryptophan and a good energy donor to fluorescein. Used to monitor release of thiols and to distinguish proliferating cancer cells by nucleolar protein staining.
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Purity
> 95% -
CAS Number
76877-33-3 -
Chemical structure
Properties
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Excitation
391nm -
Emission
472nm -
Chemical name
7-Diethylamino-3-(4-maleimidylphenyl)-4-methylcoumarin -
Molecular weight
402.44 -
Molecular formula
C24H22N2O4 -
Storage instructions
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
Solubility overview
Soluble in DMSO -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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Source
Synthetic