Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NUP155 antibody [EPR17111] (ab199528)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17111] to NUP155
  • Suitable for: WB, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-NUP155 antibody [EPR17111]
    See all NUP155 primary antibodies

  • Description

    Rabbit monoclonal [EPR17111] to NUP155

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat, HEK293 and HeLa cells lysates; Human fetal liver tissue lysate. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-NUP155 antibody [EPR17111] (ab199528)
    Western blot - Anti-NUP155 antibody [EPR17111] (ab199528)
    All lanes : Anti-NUP155 antibody [EPR17111] (ab199528) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) cell lysate
    Lane 2 : HEK293 (Human embryonic kidney) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 155 kDa
    Observed band size: 155 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP155 antibody [EPR17111] (ab199528)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP155 antibody [EPR17111] (ab199528)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling NUP155 with ab199528 at 1/2000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nucleus membrane and cytoplasm staining on HeLa cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1: ab199528 at 1/2000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow Cytometry - Anti-NUP155 antibody [EPR17111] (ab199528)
    Flow Cytometry - Anti-NUP155 antibody [EPR17111] (ab199528)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling NUP155 with ab199528 at 1/250 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • Western blot - Anti-NUP155 antibody [EPR17111] (ab199528)
    Western blot - Anti-NUP155 antibody [EPR17111] (ab199528)
    Anti-NUP155 antibody [EPR17111] (ab199528) at 1/2000 dilution + HeLa (Human epithelial cells from cervix adenocarcinoma) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 155 kDa
    Observed band size: 155 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NUP155 antibody [EPR17111] (ab199528)
    Western blot - Anti-NUP155 antibody [EPR17111] (ab199528)
    Anti-NUP155 antibody [EPR17111] (ab199528) at 1/1000 dilution + Human fetal liver lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 155 kDa
    Observed band size: 155 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-NUP155 antibody [EPR17111] (ab199528)
    Anti-NUP155 antibody [EPR17111] (ab199528)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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