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Signal Transduction Protein Trafficking Vesicle Transport Regulation

Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)

Price and availability

237 878 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671]
    See all PRK3+PRK2+PRK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR5671] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
  • Host species

    Rabbit
  • Specificity

    This antibody only detects PKN3 phosphorylated at Threonine 718, PKN2 phosphorylated at Threonine 816, and PKN1 phosphorylated at Threonine 774.
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to residues surrounding Threonine 718 of Human PKN3 (Q6P5Z2), Threonine 816 of Human PKN2 (Q16513), and Threonine 774 of Human PKN1 (Q16512) proteins.

  • Positive control

    • HeLa cell lysates and Hek 293 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR5671
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • Translation
    • Regulation
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • Other Kinases
    • Epigenetics and Nuclear Signaling
    • RNAi
    • Eukaryotic Initiation factors (eIF's)
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other

Images

  • Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)
    Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)
    All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709) at 1/1000 dilution

    Lane 1 : HeLa cell lysate untreated
    Lane 2 : HeLa cell lysate treated with Okadaic acid and Calyculin A.

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 104, 112, 99 kDa



    Bottom blot was probed with an anti-PNK2 antibody.
  • Flow Cytometry - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)
    Flow Cytometry - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)
    ab124709, at 1/10, staining PKN1 (phospho T774) + PKN2 (phospho T816) + PKN3 (phospho T718) in permeabilized Hek 293 cells by Flow cytometry.
    Green: untreated cells
    Red: Okadaic Acid + Calyculin A-treated cells
    Blue: untreated cells preincubated with the immunizing phosphopeptides
    Orange: Okadaic Acid + Calyculin A-treated cells preincubated with the immunizing phosphopeptides .
  • Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)
    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR5671] (ab124709)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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