Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1044(2)] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)
  • Suitable for: IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)]
    See all PRK3+PRK2+PRK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR1044(2)] to PRK3+PRK2+PRK1 (phospho T718 + T774 + T816)

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Lysate from Hela cells treated with Okadaic acid + Calyculin A; Human astrocytoma and colon tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Western blot - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    All lanes : Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660) at 1/10000 dilution

    Lane 1 : Lysate from Hela cells treated with Okadaic acid + Calyculin A
    Lane 2 : Untreated Hela cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 99, 112, 104 kDa



    Based on the sequence analysis, ab187660 recognizes three proteins with the predicted MWs of 99KDa, 112KDa and 104KDa, respectively.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

    Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) with ab187660 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) with ab187660 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Flow Cytometry - Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

    Flow cytometric analysis of permeabilized HeLa cells, untreated (green) or Okadaic acid + Calyculin A-treated (red) using ab187660 at 1/280 dilution, and Okadaic acid + Calyculin A-treated HeLa cells using ab187660 preincubated with phospho-PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) peptide (blue) or non-phospho-PRK3+PRK2+PRK1 (phospho T718 + T816 + T774) peptide (orange). Cells were fixed with 2% paraformaldehyde. Secondary antibody was a Goat anti IgG (FITC) at 1/150 dilution.

  • Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)
    Anti-PRK3+PRK2+PRK1 (phospho T718 + T774 + T816) antibody [EPR1044(2)] (ab187660)

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