Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [6G3] to PPP2R1A - BSA and Azide free
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPP2R1A antibody [6G3] - BSA and Azide free
    See all PPP2R1A primary antibodies

  • Description

    Rat monoclonal [6G3] to PPP2R1A - BSA and Azide free

  • Host species

    Rat

  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Other Immunogen Type within Human PPP2R1A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P30153

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, Jurkat ,Ramos, HepG2, C6 and RAW 264.7 whole cell lysate. ICC/IF: HeLa, RAW 264.7 and C6 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab255976 is the carrier-free version of ab24736. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Ion Exchange Chromatography

  • Clonality

    Monoclonal

  • Clone number

    6G3

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

    This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.

  • Western blot - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)
    Western blot - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)
    All lanes : Anti-PPP2R1A antibody [6G3] (ab24736) at 0.785 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 3 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
    Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 65 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on C6 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

    This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on RAW 264.7 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

    This image was produced using the same antibody clone but in a different formulation ab24736, PBS, sodium azide, glycerol and BSA.

  • Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)
    Anti-PPP2R1A antibody [6G3] - BSA and Azide free (ab255976)

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