Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GC-C antibody [EPR22056-278] (ab225864)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22056-278] to GC-C
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Rat

Overview

  • Product name

    Anti-GC-C antibody [EPR22056-278]
    See all GC-C primary antibodies

  • Description

    Rabbit monoclonal [EPR22056-278] to GC-C

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Mouse
    Rat
    WB
    Mouse
    Rat
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse spleen and colon lysates; Rat colon lysate. IHC-P: Mouse and rat colon tissues.

  • General notes

    Previously labelled as GUCY2C. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22056-278

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (ab225864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (ab225864)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelium of mouse colon (PMID: 27246004) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-GC-C antibody [EPR22056-278] (ab225864)
    Western blot - Anti-GC-C antibody [EPR22056-278] (ab225864)
    All lanes : Anti-GC-C antibody [EPR22056-278] (ab225864) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse colon lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 123 kDa
    Observed band size: 145,90 kDa
    why is the actual band size different from the predicted?



    Exposure time: Lane 1: 3 minutes; Lane 2: 26 seconds.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    GC-C migrates as a glycosylated 145 kDa protein and a 90 kDa cleavage product (PMID: 9344852; PMID: 26986842).

    Negative control: mouse spleen.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (ab225864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (ab225864)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining in epithelium of rat colon (PMID: 27246004) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (ab225864)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GC-C antibody [EPR22056-278] (ab225864)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling GC-C with ab225864 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Counter stained with hematoxylin.

    Negative control: no staining on mouse spleen (PMID: 26986842) is observed.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Western blot - Anti-GC-C antibody [EPR22056-278] (ab225864)
    Western blot - Anti-GC-C antibody [EPR22056-278] (ab225864)
    Anti-GC-C antibody [EPR22056-278] (ab225864) at 1/1000 dilution + Rat colon lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 123 kDa
    Observed band size: 130,145 kDa why is the actual band size different from the predicted?


    Exposure time: 37 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    GC-C migrates as two differentially glycosylated proteins with molecular masses of 130 and 145 kDa (PMID: 24217248).

  • Anti-GC-C antibody [EPR22056-278] (ab225864)
    Anti-GC-C antibody [EPR22056-278] (ab225864)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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