Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse GC-C ELISA Kit (ab246544)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 11 pg/ml
  • Range: 62.5 pg/ml - 4000 pg/ml
  • Sample type: Cell culture extracts, Tissue Extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse GC-C ELISA Kit
    See all GC-C kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Extract 8 2.6%
    Inter-assay
    Sample n Mean SD CV%
    Extract 3 3.5%

  • Sample type

    Cell culture extracts, Tissue Extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    11 pg/ml

  • Range

    62.5 pg/ml - 4000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Tissue Extracts 91 84% - 99%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse GC-C ELISA Kit (ab246544) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GC-C protein in cell culture extracts and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse GC-C with 11 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse GC-C Capture Antibody 1 x 600µl
    10X Mouse GC-C Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml
    Antibody Diluent 5BR 1 x 6ml
    Mouse GC-C Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Receptor for the E.coli heat-stable enterotoxin (E.coli enterotoxin markedly stimulates the accumulation of cGMP in mammalian cells expressing GC-C). Also activated by the endogenous peptide guanylin.

  • Sequence similarities

    Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.
    Contains 1 guanylate cyclase domain.
    Contains 1 protein kinase domain.

  • Domain

    The protein kinase domain is predicted to be catalytically inactive.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Alternative names

    • GC-C
    • Guanylyl cyclase C
    • GUC2C
    • GUC2C_HUMAN
    • GUCY2C
    • Heat stable enterotoxin receptor
    • Heat-stable enterotoxin receptor
    • hSTAR
    • Intestinal guanylate cyclase
    • STA receptor
    see all

  • Database links

    Images

    • Other - Mouse GC-C ELISA Kit (ab246544)
      Other - Mouse GC-C ELISA Kit (ab246544)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse GC-C standard curve in 1X Cell Extraction buffer PTR.
      Example of mouse GC-C standard curve in 1X Cell Extraction buffer PTR.

      The GC-C standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native GC-C in mouse small intestine and colon extract.
      Interpolated concentrations of native GC-C in mouse small intestine and colon extract.

      The concentrations of GC-C were measured in duplicate and interpolated from the GC-C standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

       

       

    • Sandwich ELISA - Mouse GC-C ELISA Kit (ab246544)
      Sandwich ELISA - Mouse GC-C ELISA Kit (ab246544)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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