Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µg 307 566 ₸ 10 µg 123 964 ₸

Order now and get it on Tuesday March 09, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rat monoclonal [6G3] to PPP2R1A
Suitable for: ICC/IF, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-PPP2R1A antibody [6G3]
See all PPP2R1A primary antibodies
Description

Rat monoclonal [6G3] to PPP2R1A
Host species

Rat

Tested Applications & Species

Application	Species
ICC/IF	Mouse Rat Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Other Immunogen Type within Human PPP2R1A. The exact sequence is proprietary.
Sequence:
Human A (subunit 7.6 G3)

Database link: P30153
Run BLAST with Run BLAST with
Positive control
- WB: HeLa, Jurkat ,Ramos, HepG2, C6 and RAW 264.7 whole cell lysate. ICC/IF: HeLa, RAW 264.7 and C6 cells.
General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Ion Exchange Chromatography
Clonality

Monoclonal
Clone number

6G3
Isotype

IgG2a
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor^® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor^®488 Goat anti-rat secondary at 1/1000 dilution.
Western blot - Anti-PPP2R1A antibody [6G3] (ab24736)

All lanes : Anti-PPP2R1A antibody [6G3] (ab24736) at 0.785 µg/ml

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

Predicted band size: 65 kDa

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor^® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on C6 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor^®488 Goat anti-rat secondary at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor^® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on RAW 264.7 cell line. The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 594) (ab150080) secondary antibody at 1/1000 dilution.
-ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor^®488 Goat anti-rat secondary at 1/1000 dilution.
Anti-PPP2R1A antibody [6G3] (ab24736)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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