Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPP2R1A antibody [6G3] (ab24736)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rat monoclonal [6G3] to PPP2R1A
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PPP2R1A antibody [6G3]
    See all PPP2R1A primary antibodies

  • Description

    Rat monoclonal [6G3] to PPP2R1A

  • Host species

    Rat

  • Tested Applications & Species

    Application Species
    ICC/IF
    Mouse
    Rat
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Other Immunogen Type within Human PPP2R1A. The exact sequence is proprietary.
    Sequence:

    Human A (subunit 7.6 G3)


    Database link: P30153
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HeLa, Jurkat ,Ramos, HepG2, C6 and RAW 264.7 whole cell lysate. ICC/IF: HeLa, RAW 264.7 and C6 cells.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

  • Western blot - Anti-PPP2R1A antibody [6G3] (ab24736)
    Western blot - Anti-PPP2R1A antibody [6G3] (ab24736)
    All lanes : Anti-PPP2R1A antibody [6G3] (ab24736) at 0.785 µg/ml

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 3 : Ramos (Human Burkitt’s lymphoma B lymphocyte), whole cell lysate
    Lane 4 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 5 : C6 (rat glial tumor glial cell), whole cell lysate
    Lane 6 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rat IgG H&L (HRP) (ab205720) at 1/10000 dilution

    Predicted band size: 65 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (rat glial tumor glial cell) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on C6 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)
    Immunocytochemistry/ Immunofluorescence - Anti-PPP2R1A antibody [6G3] (ab24736)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling PPP2R1A with ab24736 at 7.85µg/ml, followed by Goat Anti-Rat IgG (Alexa Fluor® 488) (ab150157) secondary antibody at 1/1000 dilution (green). Confocal image showing mainly showing cytoplasmic staining on RAW 264.7 cell line. The nuclear counterstain is DAPI (blue).
    Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker  at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution (red).
    The negative controls are as follows:
    -ve control 1: ab24736 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 594) (ab150080) secondary antibody at 1/1000 dilution.
    -ve control 2: ab179504 at 1/200 dilution, followed by ab150157 AlexaFluor®488 Goat anti-rat secondary at 1/1000 dilution.

  • Anti-PPP2R1A antibody [6G3] (ab24736)
    Anti-PPP2R1A antibody [6G3] (ab24736)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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