Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

Overview

  • Product name

    Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)]
    See all PPAR alpha + PPAR beta/delta primary antibodies

  • Description

    Rabbit monoclonal [EPR16673(2)] to PPAR alpha + PPAR beta/delta

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human PPAR alpha and delta full length recombinant proteins; Human fetal liver, fetal heart and fetal kidney lysates; Human skeletal muscle lysate; Jurkat, HeLa, K562, HepG2, C6, RAW264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse placenta, brain, heart, kidney and spleen lysates; Rat placenta, brain, heart and kidney lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/10000 dilution

    Lane 1 : Human PPAR alpha full length recombinant protein
    Lane 2 : Human PPAR beta full length recombinant protein
    Lane 3 : Human PPAR gamma fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 10 seconds; Lane 2: 1 second; Lane 3: 3 minutes.

    Human PPAR alpha full length recombinant protein contains aa1-468 with a His-Tag; Human PPAR beta full length recombinant protein contains aa1-441 with a His-Tag; Human PPAR gamma fragment recombinant protein contains aa1-200 with a His-Tag. These three recombinant proteins were made in-house.

  • Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Human fetal liver lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?


    Exposure time: 2 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Human skeletal muscle lysate
    Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 5 : Mouse placenta lysate
    Lane 6 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 7 : Rat placenta lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3,4 and 5: 2 seconds; Lane 6: 1 second; Lane 7: 30 seconds.

  • Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    Western blot - Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    All lanes : Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865) at 1/2000 dilution

    Lane 1 : Mouse brain lysate at 10 µg
    Lane 2 : Mouse heart lysate at 10 µg
    Lane 3 : Mouse kidney lysate at 10 µg
    Lane 4 : Mouse spleen lysate at 10 µg
    Lane 5 : Rat brain lysate at 10 µg
    Lane 6 : Rat heart lysate at 10 µg
    Lane 7 : Rat kidney lysate at 10 µg
    Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate at 20 µg
    Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg
    Lane 10 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
    Lane 11 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 52, 50 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1,2,3 and 4: 2 seconds; Lane 5,6,7,8,9,10 and 11: 1 second.

  • Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)
    Anti-PPAR alpha + PPAR beta/delta antibody [EPR16673(2)] (ab178865)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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