Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-17RA Receptor antibody [EPR22831-5]
    See all IL-17RA Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1, THP-1 and K-562 lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22831-5

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
    Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
    All lanes : Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : IL-17RA Receptor knockout HAP1 whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate
    Lane 5 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 96 kDa
    Observed band size: 120-170 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    ab263908 was shown to specifically react with IL-17RA Receptor in wild-type HAP1 cells as signal was lost in IL-17RA Receptor knockout cells. Wild-type and IL-17RA Receptor knockout samples were subjected to SDS-PAGE. ab263908 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30604628)

    Exposure time: 37 seconds

  • Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
    Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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