Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
![Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)](https://www.abcam.com/ps/products/263/ab263908/Images/ab263908-366154-anti-il-17ra-receptor-antibody-epr22831-5-western-blot-human.jpg "Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-IL-17RA Receptor antibody [EPR22831-5]
See all IL-17RA Receptor primary antibodies -
Description
Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Wild-type HAP1, THP-1 and K-562 lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22831-5 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)All lanes : Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : IL-17RA Receptor knockout HAP1 whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 4 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate
Lane 5 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 96 kDa
Observed band size: 120-170 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
ab263908 was shown to specifically react with IL-17RA Receptor in wild-type HAP1 cells as signal was lost in IL-17RA Receptor knockout cells. Wild-type and IL-17RA Receptor knockout samples were subjected to SDS-PAGE. ab263908 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30604628)
Exposure time: 37 seconds
-
![Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)](https://www.abcam.com/ps/products/263/ab263908/Images/ab263908-2-benefits-of-recombinant-antibodies.png)
Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908)
