Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-IL-17RA Receptor antibody [EPR22831-5] - BSA and Azide free (ab267402)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-IL-17RA Receptor antibody [EPR22831-5] - BSA and Azide free
    See all IL-17RA Receptor primary antibodies

  • Description

    Rabbit monoclonal [EPR22831-5] to IL-17RA Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HAP1, THP-1 and K-562 lysates.

  • General notes

    ab267402 is the carrier-free version of ab263908. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] - BSA and Azide free (ab267402)
    Western blot - Anti-IL-17RA Receptor antibody [EPR22831-5] - BSA and Azide free (ab267402)
    All lanes : Anti-IL-17RA Receptor antibody [EPR22831-5] (ab263908) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : IL-17RA Receptor knockout HAP1 whole cell lysate
    Lane 3 : THP-1 (human monocytic leukemia monocyte) whole cell lysate
    Lane 4 : MOLT-4 (human lymphoblastic leukemia t lymphoblast) whole cell lysate
    Lane 5 : K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 96 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    ab263908 was shown to specifically react with IL-17RA Receptor in wild-type HAP1 cells as signal was lost in IL-17RA Receptor knockout cells. Wild-type and IL-17RA Receptor knockout samples were subjected to SDS-PAGE. ab263908 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging. The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument using the ECL technique.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 30604628)

    Exposure time: 37 seconds

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263908).

  • Anti-IL-17RA Receptor antibody [EPR22831-5] - BSA and Azide free (ab267402)
    Anti-IL-17RA Receptor antibody [EPR22831-5] - BSA and Azide free (ab267402)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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