Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11787(B)] to PP2A alpha + beta - BSA and Azide free
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free
    See all PP2A alpha + beta primary antibodies

  • Description

    Rabbit monoclonal [EPR11787(B)] to PP2A alpha + beta - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 100% homology with PP2A beta.

  • Tested applications

    Suitable for: ICC, WBmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human PP2A alpha + beta. The exact sequence is proprietary.

  • General notes

    Ab249449 is the carrier-free version of ab168350. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249449 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    Western blot - Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution

    Lane 1 : Human PP2A beta full length recombinant protein
    Lane 2 : Human PP2A alpha full length recombinant protein

    Lysates/proteins at 0.015 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 36 kDa
    Observed band size: 59 kDa
    why is the actual band size different from the predicted?


    Exposure time: 15 seconds


    This data was developed using ab168350, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Human PP2A beta full length recombinant protein contains aa1-309 with a GST-Tag (ab128562).

    Human PP2A alpha full length recombinant protein contains aa1-309 with a GST-Tag (ab128557).

  • Western blot - Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    Western blot - Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 36 kDa



    This data was developed using ab168350, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    Immunocytochemistry - Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    This data was developed using ab168350, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of A431 cells labeling PP2A alpha + beta with ab168350 at 1/50 dilution.
  • Western blot - Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    Western blot - Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/10000 dilution + immunoprecipitation pellet from HeLa cell lysate

    Secondary
    HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

    Predicted band size: 36 kDa



    This data was developed using ab168350, the same antibody clone in a different buffer formulation.

     

  • Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
    Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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