Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free (ab249449)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11787(B)] to PP2A alpha + beta - BSA and Azide free
- Suitable for: ICC, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PP2A alpha + beta antibody [EPR11787(B)] - BSA and Azide free
See all PP2A alpha + beta primary antibodies -
Description
Rabbit monoclonal [EPR11787(B)] to PP2A alpha + beta - BSA and Azide free -
Host species
Rabbit -
Specificity
The immunogen used for this product shares 100% homology with PP2A beta.
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Tested applications
Suitable for: ICC, WBmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human PP2A alpha + beta. The exact sequence is proprietary.
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General notes
Ab249449 is the carrier-free version of ab168350. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249449 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11787(B) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/20000 dilution
Lane 1 : Human PP2A beta full length recombinant protein
Lane 2 : Human PP2A alpha full length recombinant protein
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 59 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using ab168350, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Human PP2A beta full length recombinant protein contains aa1-309 with a GST-Tag (ab128562).
Human PP2A alpha full length recombinant protein contains aa1-309 with a GST-Tag (ab128557).
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All lanes : Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 36 kDaThis data was developed using ab168350, the same antibody clone in a different buffer formulation.
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Anti-PP2A alpha + beta antibody [EPR11787(B)] (ab168350) at 1/10000 dilution + immunoprecipitation pellet from HeLa cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 36 kDaThis data was developed using ab168350, the same antibody clone in a different buffer formulation.
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