Anti-PMPCA/INPP5E antibody (ab241368)
Key features and details
- Rabbit polyclonal to PMPCA/INPP5E
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PMPCA/INPP5E antibody
See all PMPCA/INPP5E primary antibodies -
Description
Rabbit polyclonal to PMPCA/INPP5E -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide within Human PMPCA/INPP5E aa 25-75. The exact sequence is proprietary.
Database link: Q10713 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: HEK-293T whole cell lysate.
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General notes
This product was previously labelled as PMPCA
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7-8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to PMPCA/INPP5E immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PMPCA/INPP5E antibody (ab241368)All lanes : Anti-PMPCA/INPP5E antibody (ab241368) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 58 kDa
Exposure time: 30 secondsLysates prepared in NETN buffer.
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Immunoprecipitation - Anti-PMPCA/INPP5E antibody (ab241368)PMPCA/INPP5E was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded, prepared using NETN lysis buffer) using ab241368 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241368 at 1 µg/ml.
Lane 1: ab241368 IP in HEK-293T whole cell lysate.
lane 2: Control IgG IP in HEK-293T whole cell lysate.Detection: Chemiluminescence with an exposure time of 3 minutes.
