Anti-Plexin A4 antibody (ab39350)
Key features and details
- Rabbit polyclonal to Plexin A4
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Plexin A4 antibody
See all Plexin A4 primary antibodies -
Description
Rabbit polyclonal to Plexin A4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Mouse Plexin A4 aa 500-600 conjugated to keyhole limpet haemocyanin.
(Peptide available asab39349)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab39350 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 213 kDa (predicted molecular weight: 213 kDa). ICC/IF Use a concentration of 1 µg/ml. Target
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Function
Forms a receptor complex with neuropilin-1 and can propagate semaphorin-3A elicited inhibitory signals into cells and neurons. -
Sequence similarities
Belongs to the plexin family.
Contains 4 IPT/TIG domains.
Contains 3 PSI domains.
Contains 1 Sema domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 91584 Human
- Entrez Gene: 243743 Mouse
- Entrez Gene: 312213 Rat
- Omim: 604280 Human
- SwissProt: Q9HCM2 Human
- SwissProt: Q80UG2 Mouse
- Unigene: 511454 Human
- Unigene: 444029 Mouse
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Alternative names
- FAYV2820 antibody
- KIAA1550 antibody
- PLEXA4 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Plexin A4 antibody (ab39350)ICC/IF image of ab39350 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39350, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - Anti-Plexin A4 antibody (ab39350)All lanes : Anti-Plexin A4 antibody (ab39350) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/35000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 213 kDa
Observed band size: 213 kDa
Additional bands at: 190 kDa, 41 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab39350 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Western blot - Anti-Plexin A4 antibody (ab39350)All lanes : Anti-Plexin A4 antibody (ab39350) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lane 4 : Brain (Mouse) Tissue Lysate with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
Lane 5 :Mouse brain tissue lysate - total protein (0 days) (ab7188) with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
Lane 6 : Dorsal Root Ganglia (Mouse) Tissue Lysate with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Lanes 3-6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 213 kDaWe are unsure as to the biological basis of the band at 190 kDa. However, it is successfully blocked by peptide (ab39349) suggesting this may be a possible isoform of Plexin A4.
Protocols
Datasheets and documents
References (8)
ab39350 has been referenced in 8 publications.
- Liu X et al. lncRNAPCAT29 inhibits pulmonary fibrosis via the TGF-ß1-regulated RASAL1/ERK1/2 signal pathway. Mol Med Rep 17:7781-7788 (2018). PubMed: 29620190
- Lee FH et al. Disrupting GluA2-GAPDH Interaction Affects Axon and Dendrite Development. Sci Rep 6:30458 (2016). IHC-P ; Mouse . PubMed: 27461448
- Yamashita N et al. Plexin-A4-dependent retrograde semaphorin 3A signalling regulates the dendritic localization of GluA2-containing AMPA receptors. Nat Commun 5:3424 (2014). PubMed: 24599038
- Mlechkovich G et al. Distinct cytoplasmic domains in Plexin-A4 mediate diverse responses to semaphorin 3A in developing mammalian neurons. Sci Signal 7:ra24 (2014). PubMed: 24619647
- Oeschger FM et al. Gene expression analysis of the embryonic subplate. Cereb Cortex 22:1343-59 (2012). PubMed: 21862448
- Gutekunst CA et al. PlexinA4 distribution in the adult rat spinal cord and dorsal root ganglia. J Chem Neuroanat 44:1-13 (2012). PubMed: 22465808
- Gutekunst CA et al. Immunohistochemical Distribution of PlexinA4 in the Adult Rat Central Nervous System. Front Neuroanat 4: (2010). WB, ICC/IF, IHC-FoFr ; Rat . PubMed: 20700382
- Carcea I et al. Flotillin-mediated endocytic events dictate cell type-specific responses to semaphorin 3A. J Neurosci 30:15317-29 (2010). ICC/IF ; Rat . PubMed: 21068336
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Plexin A4 antibody (ab39350)ICC/IF image of ab39350 stained human SH-SY5Y cells. The cells were methanol fixed (5 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab39350, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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Western blot - Anti-Plexin A4 antibody (ab39350)All lanes : Anti-Plexin A4 antibody (ab39350) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Brain (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/35000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 213 kDa
Observed band size: 213 kDa
Additional bands at: 190 kDa, 41 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab39350 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
-
Western blot - Anti-Plexin A4 antibody (ab39350)All lanes : Anti-Plexin A4 antibody (ab39350) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 :Mouse brain tissue lysate - total protein (0 days) (ab7188)
Lane 3 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lane 4 : Brain (Mouse) Tissue Lysate with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
Lane 5 :Mouse brain tissue lysate - total protein (0 days) (ab7188) with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
Lane 6 : Dorsal Root Ganglia (Mouse) Tissue Lysate with Mouse Plexin A4 peptide (ab39349) at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Lanes 3-6 : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 213 kDaWe are unsure as to the biological basis of the band at 190 kDa. However, it is successfully blocked by peptide (ab39349) suggesting this may be a possible isoform of Plexin A4.
