Mouse RGMA ELISA Kit (ab243687)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 90 pg/ml
- Range: 0.31 ng/ml - 20 ng/ml
- Sample type: Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse RGMA ELISA Kit
See all Repulsive Guidance Molecule A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% serum 8 7% Inter-assay Sample n Mean SD CV% serum 3 8.1% -
Sample type
Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
90 pg/ml -
Range
0.31 ng/ml - 20 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 110 105% - 115% Hep Plasma 108 105% - 114% EDTA Plasma 112 105% - 121% Cit plasma 115 113% - 120% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse RGMA ELISA Kit (ab243687) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RGMA protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse RGMA with 90 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse RGMA Capture Antibody 1 x 600µl 10X Mouse RGMA Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CPR 1 x 6ml Mouse RGMA Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent 50BP 1 x 20ml Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS1-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8. -
Sequence similarities
Belongs to the repulsive guidance molecule (RGM) family. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- Repulsive guidance molecule A
- RGM
- RGM domain family member A
see all -
Database links
- Entrez Gene: 244058 Mouse
- SwissProt: Q6PCX7 Mouse
- Unigene: 333943 Mouse
Images
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Other - Mouse RGMA ELISA Kit (ab243687)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Mouse RGMA Standard Curve Comparison.Standard Curve comparison between mouse RGMA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
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Example of mouse RGMA standard curve in Sample Diluent 50BP.The RGMA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native RGMA in mouse serum and plasmas.The concentrations of RGMA were measured in duplicates, interpolated from the RGMA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 25%, plasma (citrate) 25% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RGMA concentration was determined to be 24.1 ng/mL in serum, 15.5 ng/mL in plasma (EDTA), 17.3 ng/mL in plasma (citrate) and 23.8 ng/mL plasma (heparin).
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Interpolated concentrations of native RGMA in rat serum and plasmas.The concentrations of RGMA were measured in duplicates, interpolated from the RGMA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 25%, plasma (EDTA) 50%, plasma (citrate) 25% and plasma (heparin) 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RGMA concentration was determined to be 5.3 ng/mL in serum, 4.2 ng/mL in plasma (EDTA), 4.1 ng/mL in plasma (citrate) and 5.3 ng/mL plasma (heparin).
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Sandwich ELISA - Mouse RGMA ELISA Kit (ab243687)To learn more about the advantages of recombinant antibodies see here.
