Anti-PKC epsilon (phospho S729) antibody (ab63387)
Key features and details
- Rabbit polyclonal to PKC epsilon (phospho S729)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PKC epsilon (phospho S729) antibody
See all PKC epsilon primary antibodies -
Description
Rabbit polyclonal to PKC epsilon (phospho S729) -
Host species
Rabbit -
Specificity
Detects endogenous levels of PKC epsilon only when phosphorylated at serine 729. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human PKC epsilon. Synthetic phosphopeptide from around the phosphorylation site of serine 729 (GFSPYF) of human PKC epsilon
Database link: Q02156 -
Positive control
- WB: HeLa cells treated with PMA (125ng/ml, 30mins).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PKC epsilon (phospho S729) antibody (ab63387)All lanes : Anti-PKC epsilon (phospho S729) antibody (ab63387) at 1/500 dilution
Lane 1 : HeLa cells treated with PMA (125ng/ml, 30mins) at 30 µg
Lane 2 : Jurkat cells treated eith PMA (125ng/ml, 30mins) at 30 µg
Lane 3 : NIH/3T3 cells treated with PMA (125ng/ml, 30mins) at 30 µg
Lane 4 : NIH/3T3 cells treated with syntesized peptide (125ng/ml, 30mins) at 10 µg
Predicted band size: 84 kDa
Observed band size: 84 kDa