Anti-PIWIL4/PIWI antibody (ab21869)
Key features and details
- Rabbit polyclonal to PIWIL4/PIWI
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PIWIL4/PIWI antibody
See all PIWIL4/PIWI primary antibodies -
Description
Rabbit polyclonal to PIWIL4/PIWI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Mouse PIWIL4/PIWI aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab23535) -
Positive control
- Mouse testis lysate This antibody gave a positive signal in the following Formaldehyde fixed cell line: HeLa.
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General notes
This product was previously labelled as PIWIL4
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Coimmunogen
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21869 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species ICC/IF HumanWB MouseAll applications RatApplication Abreviews Notes ICC/IF Use a concentration of 5 µg/ml.WB (1) Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). Block with 5% BSA for 1 hour and incubate antibody in TBST for 1 hour or more.Notes ICC/IF
Use a concentration of 5 µg/ml.WB
Use a concentration of 1 µg/ml. Detects a band of approximately 98 kDa (predicted molecular weight: 98 kDa). Block with 5% BSA for 1 hour and incubate antibody in TBST for 1 hour or more.Target
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Function
Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci. -
Tissue specificity
Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed. -
Sequence similarities
Belongs to the argonaute family. Piwi subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Post-translational
modificationsArginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule. -
Cellular localization
Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization. - Information by UniProt
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Database links
- Entrez Gene: 143689 Human
- Entrez Gene: 330890 Mouse
- Entrez Gene: 689972 Rat
- Omim: 610315 Human
- SwissProt: Q7Z3Z4 Human
- SwissProt: Q8CGT6 Mouse
- SwissProt: Q4G033 Rat
- Unigene: 660188 Human
see all -
Alternative names
- DKFZp686P01248 antibody
- FLJ36156 antibody
- HILI 2 antibody
see all
Images
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Western blot - Anti-PIWIL4/PIWI antibody (ab21869)
ab21869 specifically recognises PIWIL4/PIWI at 98kDa, which is demonstrated by the efficient blocking by the immunizing peptide (ab23535).
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Immunocytochemistry/ Immunofluorescence - Anti-PIWIL4/PIWI antibody (ab21869)ICC/IF image of ab21869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab21869 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References (5)
ab21869 has been referenced in 5 publications.
- Nishimura T et al. PNLDC1, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep 19:N/A (2018). Mouse . PubMed: 29444933
- Heng ZSL et al. The role of 17ß-estradiol-induced upregulation of Piwi-like 4 in modulating gene expression and motility in breast cancer cells. Oncol Rep 40:2525-2535 (2018). PubMed: 30226541
- Ding D et al. PNLDC1 is essential for piRNA 3' end trimming and transposon silencing during spermatogenesis in mice. Nat Commun 8:819 (2017). IHC-P ; Mouse . PubMed: 29018194
- Keam SP et al. The human Piwi protein Hiwi2 associates with tRNA-derived piRNAs in somatic cells. Nucleic Acids Res 42:8984-95 (2014). WB, ICC/IF ; Human . PubMed: 25038252
- Yabuta Y et al. TDRD5 is required for retrotransposon silencing, chromatoid body assembly, and spermiogenesis in mice. J Cell Biol 192:781-95 (2011). IHC-Fr ; Mouse . PubMed: 21383078
Images
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Western blot - Anti-PIWIL4/PIWI antibody (ab21869)
ab21869 specifically recognises PIWIL4/PIWI at 98kDa, which is demonstrated by the efficient blocking by the immunizing peptide (ab23535).
-
Immunocytochemistry/ Immunofluorescence - Anti-PIWIL4/PIWI antibody (ab21869)
ICC/IF image of ab21869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab21869 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
