Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17843] to PIGK - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PIGK antibody [EPR17843] - BSA and Azide free
    See all PIGK primary antibodies

  • Description

    Rabbit monoclonal [EPR17843] to PIGK - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab251354 is the carrier-free version of ab201693.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR17843

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    Immunocytochemistry - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)

    This data was developed using ab201693, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling PIGK with ab201693 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing cytoplasmic staining on Jurkat cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab201693 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Immunocytochemistry - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    Immunocytochemistry - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)

    This data was developed using ab201693, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling PIGK with ab201693 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green).Confocal image showing cytoplasmic staining on A549 cell line.The nuclear counter stain is DAPI (blue).Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).The negative controls are as follows:-ve control 1: ab201693 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.
  • Western blot - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    Western blot - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    All lanes : Anti-PIGK antibody [EPR17843] (ab201693) at 1/2000 dilution

    Lane 1 : HT1080 (Human fibrosarcoma cells) cell lysate
    Lane 2 : HEK-293 (Human epithelial cells from embryonic kidney) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 minute


    This data was developed using ab201693, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    Western blot - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    All lanes : Anti-PIGK antibody [EPR17843] (ab201693) at 1/2000 dilution

    Lane 1 : Human pancreas lysate
    Lane 2 : Human fetal liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 3 minutes


    This data was developed using ab201693, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    Western blot - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    All lanes : Anti-PIGK antibody [EPR17843] (ab201693) at 1/2000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse heart tissue lysate
    Lane 3 : Mouse kidney tissue lysate
    Lane 4 : Mouse spleen tissue lysate
    Lane 5 : Rat brain tissue lysate
    Lane 6 : Rat kidney tissue lysate
    Lane 7 : C6 (Rat glial tumor cells) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 45 kDa
    Observed band size: 45 kDa


    Exposure time: 1 minute


    This data was developed using ab201693, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    Flow Cytometry - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    This data was developed using ab201693, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 2% paraformaldehyde-fixed HEK-293 (Human epithelial cells from embryonic kidney) cells labeling PIGK with ab201693 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
  • Immunoprecipitation - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    Immunoprecipitation - Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    This data was developed using ab201693, the same antibody clone in a different buffer formulation.PIGK was immunoprecipitated from 1mg of HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab201693 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201693 at 1/2000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1500 dilution. Lane 1: HEK293 whole cell lysate10 µg (Input). Lane 2: ab201693 IP in HEK293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201693 in HEK293 whole cell lysate. Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure time: 5 seconds.
  • Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)
    Anti-PIGK antibody [EPR17843] - BSA and Azide free (ab251354)

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