Anti-ERp72 antibody [EPR14825] - BSA and Azide free (ab251017)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14825] to ERp72 - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-ERp72 antibody [EPR14825] - BSA and Azide free
See all ERp72 primary antibodies -
Description
Rabbit monoclonal [EPR14825] to ERp72 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab251017 is the carrier-free version of ab190354.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
EPR14825 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-ERp72 antibody [EPR14825] - C-terminal (ab190354) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : MCF7 cell lysate
Lane 4 : JAR cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 73 kDa
Observed band size: 73 kDaThis data was developed using ab190354, the same antibody clone in a different buffer formulation.
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This data was developed using ab190354, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labeling ERp72 with ab190354 at 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counterstained with Heamatoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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This data was developed using ab190354, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling ERp72 with ab190354 at 1/100 dilution. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counterstained with Heamatoxylin. Negative control also shown. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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