Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3554] to MRP8 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-MRP8 antibody [EPR3554] - BSA and Azide free
    See all MRP8 primary antibodies

  • Description

    Rabbit monoclonal [EPR3554] to MRP8 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cervical carcinoma, breast carcinoma, tonsil and spleen tissues. IP: HL-60 cell lysate.

  • General notes

    ab271863 is the carrier-free version of ab92331. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labeling MRP8 with purified ab92331 at 1/500.

    Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).
  • Immunoprecipitation - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)
    Immunoprecipitation - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)

    ab92331 (purified) at 1/30 immunoprecipitating MRP8 in HL-60 (Human promyelocytic leukemia cell line) cell lysate (Lane 1).

    Lane 2 - rabbit monoclonal IgG instead of ab92331 in HL-60 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

    Blocking/Dilution buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling MRP8 with unpurified ab92331.

    Detection: DAB staining.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling MRP8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling MRP8 with unpurified ab92331.

    Detection: DAB staining.

    Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MRP8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92331).
  • Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)
    Anti-MRP8 antibody [EPR3554] - BSA and Azide free (ab271863)

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