Antibodies, Proteins, Kits and Reagents for Life Science

338 390 ₸ Product size

100 µl 338 390 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3554] to MRP8
Suitable for: WB, IP, IHC-P
Reacts with: Mouse, Human

Product name

Anti-MRP8 antibody [EPR3554]
See all MRP8 primary antibodies
Description

Rabbit monoclonal [EPR3554] to MRP8
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human MRP8 aa 50 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P05109
Positive control
- WB: Human (adult and fetal) and mouse spleen tissue lysates and PMBC and HL-60 cell lysates. IHC-P: Human cervical carcinoma, breast carcinoma, tonsil and spleen tissues. IP: HL-60 cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3554
Isotype

IgG
Research areas

Western blot - Anti-MRP8 antibody [EPR3554] (ab92331)

All lanes : Anti-MRP8 antibody [EPR3554] (ab92331) at 1/20000 dilution (purified)

Lane 1 : HL-60 (Human promyelocytic leukemia cell line) cell lysate
Lane 2 : Human fetal spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix carcinoma tissue labeling MRP8 with purified ab92331 at 1/500.

Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunoprecipitation - Anti-MRP8 antibody [EPR3554] (ab92331)

ab92331 (purified) at 1/30 immunoprecipitating MRP8 in HL-60 (Human promyelocytic leukemia cell line) cell lysate (Lane 1).

Lane 2 - rabbit monoclonal IgG instead of ab92331 in HL-60 lysates. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1500).

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling MRP8 with unpurified ab92331.

Detection: DAB staining.

Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-MRP8 antibody [EPR3554] (ab92331)

Anti-MRP8 antibody [EPR3554] (ab92331) at 1/2000 dilution (purified) + Mouse spleen tissue lysate at 20 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 11 kDa
Observed band size: 11 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling MRP8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Western blot - Anti-MRP8 antibody [EPR3554] (ab92331)

All lanes : Anti-MRP8 antibody [EPR3554] (ab92331) (unpurified)

Lane 1 : Human spleen tissue lysate
Lane 2 : PBMC cell lysate
Lane 3 : HL-60 (Human promyelocytic leukemia cell line) cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 11 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labeling MRP8 with unpurified ab92331.

Detection: DAB staining.

Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MRP8 antibody [EPR3554] (ab92331)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling MRP8 with unpurified ab92331. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
Anti-MRP8 antibody [EPR3554] (ab92331)

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