Anti-PICK1 antibody [L20/8] (ab252543)
![Anti-PICK1 antibody [L20/8] (ab252543)](https://www.abcam.com/ps/products/252/ab252543/Images/ab252543-368228-AntiPICK1-antibody-L20-8-western-blot-hela-human.jpg "Anti-PICK1 antibody [L20/8] (ab252543)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [L20/8] to PICK1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PICK1 antibody [L20/8]
See all PICK1 primary antibodies -
Description
Mouse monoclonal [L20/8] to PICK1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- WB: HeLa whole cell lysate. Mouse and rat brain tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
L20/8 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-PICK1 antibody [L20/8] (ab252543)Anti-PICK1 antibody [L20/8] (ab252543) at 0.509 µg/ml + HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 46 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
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![Western blot - Anti-PICK1 antibody [L20/8] (ab252543)](https://www.abcam.com/ps/products/252/ab252543/Images/ab252543-368227-AntiPICK1-antibody-L20-8-western-blot-brain-mouse-rat.jpg)
Western blot - Anti-PICK1 antibody [L20/8] (ab252543)All lanes : Anti-PICK1 antibody [L20/8] (ab252543) at 0.509 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 46 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-PICK1 antibody [L20/8] (ab252543)](https://www.abcam.com/ps/products/252/ab252543/Images/ab252543-3-benefits-of-recombinant-antibodies.png)
Anti-PICK1 antibody [L20/8] (ab252543)
