Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PICK1 antibody [EPR4129(2)] (ab109249)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4129(2)] to PICK1
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-PICK1 antibody [EPR4129(2)]
    See all PICK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4129(2)] to PICK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Fetal brain, HeLa, LnCaP and SH-SY5Y cell lysates IP: HeLa whole cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-PICK1 antibody [EPR4129(2)] (ab109249)
    Western blot - Anti-PICK1 antibody [EPR4129(2)] (ab109249)
    All lanes : Anti-PICK1 antibody [EPR4129(2)] (ab109249) at 1/1000 dilution

    Lane 1 : Fetal brain tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : LnCaP cell lysate
    Lane 4 : SH SY5Y cell lysate

    Predicted band size: 47 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-PICK1 antibody [EPR4129(2)] (ab109249)
    Immunoprecipitation - Anti-PICK1 antibody [EPR4129(2)] (ab109249)

    ab109249 (purified) at 1/30 dilution (20 µg/ml) immunoprecipitating PICK1 in HeLa whole cell lysate.

    Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab109249 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109249 in HeLa whole cell lysate

    For western blotting, ab227648 (purified at 1/500 dilution (0.012 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
    Blocking and diluting buffer: 5% NFDM /TBST .

  • Anti-PICK1 antibody [EPR4129(2)] (ab109249)
    Anti-PICK1 antibody [EPR4129(2)] (ab109249)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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