Anti-PICK1 antibody [EPR4129(2)] (ab109249)
![Anti-PICK1 antibody [EPR4129(2)] (ab109249)](https://www.abcam.com/ps/products/109/ab109249/Images/ab109249-121942-anti-pick1-antibody-epr41292-western-blot.jpg "Anti-PICK1 antibody [EPR4129(2)] (ab109249)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4129(2)] to PICK1
- Suitable for: WB, IP
- Reacts with: Human
Overview
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Product name
Anti-PICK1 antibody [EPR4129(2)]
See all PICK1 primary antibodies -
Description
Rabbit monoclonal [EPR4129(2)] to PICK1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Fetal brain, HeLa, LnCaP and SH-SY5Y cell lysates IP: HeLa whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4129(2) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PICK1 antibody [EPR4129(2)] (ab109249)All lanes : Anti-PICK1 antibody [EPR4129(2)] (ab109249) at 1/1000 dilution
Lane 1 : Fetal brain tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : LnCaP cell lysate
Lane 4 : SH SY5Y cell lysate
Predicted band size: 47 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
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![Immunoprecipitation - Anti-PICK1 antibody [EPR4129(2)] (ab109249)](https://www.abcam.com/ps/products/109/ab109249/Images/ab109249-2-anti-pick1-antibody-epr4129-2-immunoprecipitation-hela-human.jpg)
Immunoprecipitation - Anti-PICK1 antibody [EPR4129(2)] (ab109249)ab109249 (purified) at 1/30 dilution (20 µg/ml) immunoprecipitating PICK1 in HeLa whole cell lysate.
Lane 1 (input): HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab109249 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109249 in HeLa whole cell lysate
For western blotting, ab227648 (purified at 1/500 dilution (0.012 µg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.
Blocking and diluting buffer: 5% NFDM /TBST . -
![Anti-PICK1 antibody [EPR4129(2)] (ab109249)](https://www.abcam.com/ps/products/109/ab109249/Images/ab109249-3-benefits-of-recombinant-antibodies.png)
Anti-PICK1 antibody [EPR4129(2)] (ab109249)
